pHPV-1 (3-3) (ATCC® 45021)

Organism: human papilloma virus type 1a  /  Clone Type: Clone  /  Depositors: PM Howley

Designations pHPV-1 (3-3)
Species human papilloma virus type 1a
Depositors PM Howley
Vector
Construct size (kb): 12.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 8.0
Tissue: DNA from human plantar wart
Gene product: human papilloma virus genome
Insert Size (kb) 8.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Shipping Information Distributed: freeze-dried
Comments
pHPV-1 (3-3) isolated by P. Howley from a plantar wart. Entire genomic DNA linearized at BamHI site into BamHI site of pBR322.
Under standard conditions, no homology is detectable between HPV-1a, HPV-1b, HPV-2a, and HPV-4.
Under less stringent conditions, the HPV DNAs and BPV-1 demonstrate about 30% base mismatch.
Circular HPV-1a was linearized with BamHI before cloning.
References

Heilman CA, et al. Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J. Virol. 36: 395-407, 1980. PubMed: 6253665

Gissmann L, et al. Human papilloma viruses (HPV): characterization of four different isolates. Virology 76: 569-580, 1977. PubMed: 65825

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