pTL-0059 (ATCC® 45035)

Organism: Tobacco etch virus  /  Clone Type: Clone  /  Depositors: WG Dougherty

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Designations pTL-0059
Species Tobacco etch virus
Depositors WG Dougherty
Construct size (kb): 8.989999771118164
Insert lengths(kb): 5.909999847412109
Gene product: VPg, 6 kDa
Insert Size (kb) 5.910
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; PstI--9.0; HindIII--5.2, 2.25, 1.1 + others; EcoRI--9.0.
The vector (pTL-8) allows in vitro expression of the insert as a fusion protein.
The insert includes TEV cDNA sequences from nt 0 to 5915.
Classification Potyvirus

Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986.

Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076

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