pHPV-16 purified plasmid DNA (ATCC® 45113D)

Organism: human papilloma virus type 16  /  Clone Type: Clone  /  Depositors: JE Marich, Syngene, Inc

Designations pHPV-16 purified plasmid DNA
GenBank Definition

Human papillomavirus type 16 (HPV16), complete genome

GenBank Number

K02718

Species human papilloma virus type 16
Depositors JE Marich, Syngene, Inc
Vector
Size of Construct (kb): 10.866
Excise insert: BamHI+BglI (insert fragment will include 205 nt of the vector).


Vector: pBluescript SK-
Size of intact vector (kb): 2.960
Markers: ampR
Vector ends: XhoI
Insert
Insert size (kb): 7.904
Genome: human papilloma virus type 16
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: genomic
Insert ends: BamHI
Insert Size (kb) 7.904
Related Products
Source culture: ATCC 45113
Biosafety Level 2
Shipping Information 200 ng purified plasmid DNA, dried. Rehydrate with TE or water.
Comments
Cloned from human papilloma virus type 16 by linearizing the virus at the unique BamHI site.
Full-length.

The insert contains the following restriction sites (nt from the vector KpnI site [nt according to GenBank accession K02718]): PstI--654 [6791], 870 [7007], 2646 [879], 5463 [3696], 6526 [4759], 7009 [5242]; KpnI--2651 [884], 7148 [5381]; EcoRI - 685 [6818], 1320 [7453].

BamHI+BglI will excise the insert with 205 nucleotides of the vector.

Restriction digests of the clone give the following sizes (kb): BamHI/BglI--8.0, 1.5, 1.2; PstI--3.6, 2.9, 1.8, 1.1, 1.0, 0.5; KpnI--4.4, 3.7, 2.7; EcoRI--7.0, 3.6, 0.6.
Classification Papovavirus, Papillomavirus, recombinant
References

James E Marich, personal communication

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