pSP65:pUAS14#4 plasmid in E. coli (ATCC® 45117)

Organism: Apple scar skin viroid  /  Clone Type: Clone  /  Depositors: AF Hadidi

Designations pSP65:pUAS14#4 plasmid in E. coli
GenBank Number

M36646

Species Apple scar skin viroid
Depositors AF Hadidi
Vector
Construct size (kb): 3.3
Insert
DNA: cDNA
Insert lengths(kb): 0.274
Insert Size (kb) 0.274
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.3; PstI--3.3; BglI--3.3; HindIII--3.3.
Derived from nucleotides 286 to 228 in the sequence of the circular viroid.
If the plasmid is linearized with PstI or HindIII, transcription with SP6 polymerase will generate the complement (minus strand).
Classification Aspcaviroid
References

Hashimoto J, Koganezawa H. Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Res. 15: 7045-7051, 1987. PubMed: 3658673

Ahmed F Hadidi, personal communication

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