pURSP2 (ATCC® 77294)

Organism: Schizosaccharomyces pombe Lindner, fission yeast  /  Clone Type: Library  /  Depositors: NC Barbet, AM Carr

Permits and Restrictions

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Designations pURSP2
Species Schizosaccharomyces pombe Lindner, fission yeast
Depositors NC Barbet, AM Carr
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUR19
Intact vector size: 5.500
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: EcoRI SacI KpnI SmaI BamHI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC19, pON163
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: ars1
replicon: pMB1
Cross references:
Vector
DNA: genomic
Digest: Partial
Insert size range: 1.5 - 5.0 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 5000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
Average insert size: 2.5 kb.
References

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

Product Sheet
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