IowaI (ATCC® 87668)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

Permits and Restrictions

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Designations IowaI
Species Cryptosporidium parvum Tyzzer
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
Tissue: sporozoites/oocysts
Number of independent recombinants: 1.7 x10(5)
Percentage non-recombinants: 15%
Titer: 3.0 x 10(8) cfu/ml
DNA: genomic
Digest: Complete
Insert size range: 0.3 - 3.0 kb
Coverage: Clones for "complete" coverage: 17000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

 Frozen stock of library in Escherichia coli.

                   

Comments
DNA was isolated from oocysts of Cryptosporidium parvum, Iowa (I) isolate, obtained from the feces of a calf near Ames Iowa in the mid-1980's and are passaged continually in calves at Pleasant Hill Farm in Idaho.
DNA was isolated from oocysts of Cryptosporidium parvum, Iowa (I) isolate, obtained from the feces of a calf near Ames, Iowa in the mid-1980's and have been passaged continually in calves in Idaho since that time.
References

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

Slapeta J. Cryptosporidium species found in cattle: a proposal for a new species. Trends Parasitol. 22: 469-474, 2006.

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