c9171 [9171] (ATCC® 70884)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Permits and Restrictions

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Designations c9171 [9171]
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Vector
Construct size (kb): 0.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: HmL
Gene name: mating-type cassette
Contains complete coding sequence?: U
Chromosome: III; Localization: III L, 61 cM from CEN
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references:
Gene product: mating-type cassette [HML]
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.542, 0.441, 2.551, 2.208, 3.547, 1.879, 0.553, 0.495, 0.916, 10.083, 2.281, 1.471, 0.769, 1.213, 1.139.
Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.35, 0.46, 0.51, 0.74, 0.89, 1.1, 1.2, 1.5, 1.9, 2.3, 2.7, 3.8, 4.9, ***.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70051, 70739.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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