pACYC177/ET3d/yNMT (ATCC® 87052)

Other IDs:

Nucleotide (GenBank) : U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.

 /  Applications: produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase  /  Depositors: RS Haun

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Designations pACYC177/ET3d/yNMT
Other IDs

Nucleotide (GenBank) : U25270 Expression cloning vector pACYC177/ET3d/yNMT, complete sequence.

Depositors RS Haun
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli BL21(DE3)
Vector Information
Size (kb): 4.9429998397827150
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACYC177/ET3d
Intact vector size:
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI, NcoI+BamHI
Polylinker sites:
Construction: pACYC177,pET3d
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: p15A
marker(s): kanR, ->
ribosome-binding site: gene 10
promoter: T7 (phi10), ->
Cross references:
Vector: pACYC177/ET3d/yNMT (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pACYC177,pET3d
Marker(s):kanR
Construct size (kb): 4.900000095367432
Features: marker(s): kanR
promoter for expression: T7 (phi10)
replicon: p15A
coding sequence: NMT1
Applications
produces protein peptide N-myristoyl transferase glycylpeptide N-tetradecanoyltransferase
Comments
Restriction digests of the clone give the following sizes (kb): NdeI--5.5; NcoI--5.5; StuI--3.9, 1.5.
Expression system compatible with ColE1 plasmids, for cotransformation and production of myristoylated proteins.
Introduction of the NcoI site during amplification introduced a single base substitution T->G at nt 4.
Expression of this gene in the presence of 100 uM myristic acid allows myristoylation of recombinant proteins produced by a second ColE1 plasmid.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Haun RS, et al. Effect of myristoylation on GTP-dependent binding of ADP-ribosylation factor to Golgi. J. Biol. Chem. 268: 7064-7068, 1993. PubMed: 8463239

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map