Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47055)

Strain Designations: S17-1  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations S17-1
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Shipping Information Frozen Escherichia coli.
Storage Conditions -80°C or colder
Preceptrol® no
Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Type Strain no
Comments
Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes.
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA.
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Name of Depositor D Parke, Department of Biology, Yale University, New Haven, CT
Chain of Custody
ATCC <-- D Parke <-- R Simon
References

McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.

Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423

Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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