Bacillus subtilis (Ehrenberg) Cohn (ATCC® 47096)

Strain Designations: BG2864  /  Type Strain: no  /  Biosafety Level: 1

Strain Designations BG2864
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype ΔaprE ΔnprE ΔflaA::kan
Type Strain no
Comments
This host has been used for transformation and screening of gene mutant libraries.
This strain was constructed by introducing the ΔflaA::kan mutation from strain PB5129 into strain BG2036.
Medium ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype ΔaprE ΔnprE ΔflaA::kan
Name of Depositor C Wang
References

Shafikhani S, et al. Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization. BioTechniques 23: 304-310, 1997. PubMed: 9266088