Primers have been tested that yield DNA fragments sizes ranging between 203 and 1455 bp.
A set of plasmids to be used as controls for detecting mutations or validating methods of mutation scanning. The mutations are either point variants (A, T, C or G at nt 272) or insertions of one to five nucleotides (starting at nt 274).
The plasmids were derived from pUC19 (GenBank M77789), with each plasmid containing a 10 to 15 base pair replacement between the BamHI and EcoRI sites. These plasmids are designed so that the same PCR primer pair can be used on each plasmid.