SRS for Detecting Protein-Protein Interactions (ATCC® 87639)

Depositors: SJ Elledge

Permits and Restrictions

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Designations SRS for Detecting Protein-Protein Interactions
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli JM107 (ATCC 47014)
Vector Information
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMS-TRP
Intact vector size: 6.828
Type of vector: phagemid
Vector end: EcoRI
Vector end: XhoI
Cloning sites: EcoRI SacI KpnI XhoI SphI
Polylinker sites: EcoRI SacI KpnI XhoI SphI
Other unique sites: HpaI SwaI mLuI NotI BglI MunI BglII
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron ori
replicon: f1
promoter: GAL, ->
promoter: T7, ->
other: Myristoylation site, ->
epitope tag: 3x hemaglutinin (HA), ->
MCS: EcoRI...SphI, ->
terminator: CYC1, ->
other: lox site
restriction site: NotI
other: lox site
replicon: pMB1
marker(s): ampR, <-
marker(s): TRP1, <-
Cross references:
References

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

Brachmann RK, Boeke JD. Tag games in yeast: the two-hybrid system and beyond. Curr. Opin. Biotechnol 8: 561-568, 1997. PubMed: 9353226

Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet