YRp17 (ATCC® 37078)

Other IDs:

Nucleotide (GenBank) : VB0069 YRp17 yeast replicating plasmid.

 /  Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: RW Davis

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Designations YRp17
Depositors RW Davis
Other IDs

Nucleotide (GenBank) : VB0069 YRp17 yeast replicating plasmid.

Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: YRp17 (plasmid)
Promoters: Promoter none
Construction: YIp5, TRP1-ARS1
Marker(s):TRP1,URA3,ampR,tetR
Construct size (kb): 7.0
Features: marker(s): ampR, tetR, URA3, TRP1
promoter: none
replicon: pMB1, ARS1
terminator: none
enhancer: none
Applications
YR-type (replicating) shuttle vector
shuttle vector
Comments
YRp17 transforms yeast at high frequencies and is mitotically unstable.
A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YRp17 differs from YRp12 by the loss of the EcoRI site adjacent to the ampR gene. YRp12 was constructed from YIp5 (ATCC 37061) by inserting a 1.45 kb EcoRI TRP1-ARS1 fragment.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Mann C, Davis RW. Instability of dicentric plasmids in yeast. Proc. Natl. Acad. Sci. USA 80: 228-232, 1983. PubMed: 6337372

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
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