YRp17 (ATCC® 37078)

Other IDs:

Nucleotide (GenBank) : VB0069 YRp17 yeast replicating plasmid.

 /  Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: RW Davis

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Designations YRp17
Depositors RW Davis
Other IDs

Nucleotide (GenBank) : VB0069 YRp17 yeast replicating plasmid.

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0000000000000000
Vector: YRp17 (plasmid)
Promoters: Promoter none
Construction: YIp5, TRP1-ARS1
Marker(s):TRP1,URA3,ampR,tetR
Construct size (kb): 7.0
Features: marker(s): ampR, tetR, URA3, TRP1
promoter: none
replicon: pMB1, ARS1
terminator: none
enhancer: none
Applications
YR-type (replicating) shuttle vector
shuttle vector
Comments
YRp17 transforms yeast at high frequencies and is mitotically unstable.
A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
YRp17 differs from YRp12 by the loss of the EcoRI site adjacent to the ampR gene. YRp12 was constructed from YIp5 (ATCC 37061) by inserting a 1.45 kb EcoRI TRP1-ARS1 fragment.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Mann C, Davis RW. Instability of dicentric plasmids in yeast. Proc. Natl. Acad. Sci. USA 80: 228-232, 1983. PubMed: 6337372

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried
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