pSP65-f1+ (ATCC® 37359)

Applications: vector permitting RNA synthesis in vitrovector permitting production of single-stranded DNA  /  Depositors: DA Mead

Permits and Restrictions

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Designations pSP65-f1+
Depositors DA Mead
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5000000000000000
Vector: pSP65-f1+ (phagemid)
Promoters: Promoter SP6
Construction: bacteriophage f1, pSP65
Construct size (kb): 3.5
Features: marker(s): ampR
promoter: SP6
replicon: pMB1, f1
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
ssDNA SP6 promoter plasmid.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795

Shipped freeze-dried