pWE16 (ATCC® 37524)

Applications: contains easily purifiable cassette(s) for constructionexpression vectorintegrating vectorshuttle vectorvector for constructing genomic librariesvector permitting RNA synthesis in vitrovector permitting positive selection for integration  /  Depositors: GA Evans

Permits and Restrictions

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Designations pWE16
Depositors GA Evans
Biosafety Level 1
Vector Information
Size (kb): 8.8000001907348630
Vector: pWE16 (cosmid)
Promoters: Promoter T3
Construction: pCV107, pCV108, pJB8
Marker(s):ampR,mtxR
Construct size (kb): 8.800000190734863
Features: marker(s): ampR, mtxR
promoter: T3, T7
replicon: pMB1
Applications
contains easily purifiable cassette(s) for construction
expression vector
integrating vector
shuttle vector
vector for constructing genomic libraries
vector permitting RNA synthesis in vitro
vector permitting positive selection for integration
Comments
pWE15 (ATCC 37503) and pWE16 (ATCC 37524) differ only in the markers selectable in vertebrate cells.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Useful for constructing genomic libraries and chromosome walking and for gene transfer using dihydrofolate reductase as a selectable marker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Wahl GM, et al. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. USA 84: 2160-2164, 1987. PubMed: 3031668

Evans GA, Wahl GM. Cosmid vectors for genomic walking and rapid restriction mapping. Methods Enzymol. 152: 604-610, 1987. PubMed: 2443810

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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