pTacNeo2.1 [CMCC 2298] (ATCC® 37689)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteins  /  Depositors: JC Hunter-Cevera

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Designations pTacNeo2.1 [CMCC 2298]
Depositors JC Hunter-Cevera
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.4400000572204590
Vector: pTacNeo2.1 (plasmid)
Promoters: Promoter tac
Construction: pBR322, TAC promoter, lacZ, modified APH(3')-I
Marker(s):ampR
Construct size (kb): 5.440000057220459
Features: marker(s): ampR
promoter: tac
replicon: pMB1
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.0, 1.5; PstI--3.1, 2.4; BamHI--5.5; SmaI--5.5; XbaI--5.5.
This is an expression vector capable of producing a B-gal: insert fusion protein. It can also be used as a probe.
The start codon of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted.
The upstream translation of LacZ is terminated before APH(3')-I.
The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I).
Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jennie C Hunter-Cevera, personal communication

Shipped freeze-dried
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