YEp357R (ATCC® 37738)

Applications: YE-type (episomal) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: CJ Lusty

Permits and Restrictions

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Designations YEp357R
Depositors CJ Lusty
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.0000000000000000
Vector: YEp357R (plasmid)
Promoters: Promoter none
Construction: YEp357 (ATCC 37732), pUC18
Marker(s):URA3,ampR
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
promoter: none
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
promoter-cloning vector
shuttle vector
Comments
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-3; SphI-1; PstI-1; SalI-3; XbaI-3; BamHI-3; SmaI-1; KpnI-1; SacI-1; EcoRI-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcorI--8.0; PstI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37737 - 37739) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'A AGC TTG CAT GCC TGC AGG TCG ACT CTA GAG GAT CCC CGG GTA CCG AGC TCG AAT TCC CAG CTT GCT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915

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