Size (kb): 8.0000000000000000
Vector: YEp357R (plasmid)
Promoters: Promoter none
Construction: YEp357 (ATCC 37732)
Construct size (kb): 8.0
Features: insert detection: lacZ
marker(s): ampR, URA3
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-3; SphI-1; PstI-1; SalI-3; XbaI-3; BamHI-3; SmaI-1; KpnI-1; SacI-1; EcoRI-3. The SacI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.0; EcorI--8.0; PstI--8.0.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37737
- 37739) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5'A AGC TTG CAT GCC TGC AGG TCG ACT CTA GAG GAT CCC CGG GTA CCG AGC TCG AAT TCC CAG CTT GCT CCC3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915