pRY253 (ATCC® 39688)

Applications: YI-type (integrating) shuttle vectorvector permitting positive selection for integration  /  Depositors: RR Yocum

Permits and Restrictions

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Designations pRY253
Depositors RR Yocum
Biosafety Level 1
Host
Distribution host: Escherichia coli YMC9
Vector Information
Size (kb): 14.0000000000000000
Vector: pRY253 (plasmid)
Promoters: Promoter CYC1
Construction: pRY253
Marker(s):ampR G418R URA3
Construct size (kb): 14.0
Features: marker(s): G418R
marker(s): URA3
marker(s): ampR
other: HO
promoter: CYC1
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
vector permitting positive selection for integration
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0, 7.0; KpnI--14.0; XbaI--uncut; SalI--10.8, 3.2; SstI--14.0.
A YI-type (yeast integrating, HO gene) shuttle vector that confers G418 resistance under control of the CYC1 promoter.
Constructed from pRY252 (ATCC 39687) by replacing the EcoRI fragment containing the 2 micron origin with an EcoRI fragment containing the homothalism gene HO and the KpnI site that can be used for cloning.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yocum RR, et al. Industrial yeast comprising an integrated glucoamylase gene. US Patent 5,422,267 dated Jun 6 1995

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet