pUFR027 (ATCC® 77121)

Applications: shuttle vectorvector permitting visual detection of recombinants  /  Depositors: DW Gabriel

Permits and Restrictions

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Designations pUFR027
Depositors DW Gabriel
Biosafety Level 1
Vector Information
Size (kb): 9.3000001907348630
Vector: pUFR027 (plasmid)
Promoters: Promoter lac
Construction: pSa747, pUCD550, pUC4Kan, pLAFR3, pUC18
Marker(s):neoR
Construct size (kb): 9.300000190734863
Features: insert detection: lacZ'
marker(s): neoR
promoter: lac
replicon: oriW
Applications
shuttle vector
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; PstI--6.8, 1.4, 1.25; SmaI--7.7, 1.9.
Uses interruption of lacZalpha.
Shuttle vector permitting visual detection of recombinants.
The partition locus from Agrobacterium plasmid pTAR stabilizes the plasmid in X. citri and X. campestris pvs. campestris, vesicatoria, malvacearum, and translucens grown in broth culture.
The order of the major features in this plasmid is: lacZalpha with MCS (pUC18) - oriW (pSa747, IncW) - parA+ (pUCD550) - aphA (pUC4Kan) - mob(P) (pLAFR3, Mob+).
Media ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions
Temperature: 37.0°C
References

DeFeyter R, et al. Small, stable shuttle vectors for use in Xanthomonas. Gene 88: 65-72, 1990. PubMed: 2341039

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet