pLDR30 [WM2190, pNON11] (ATCC® 87202)

Applications: contains easily purifiable cassette(s) for construction pMB1 ori - rop cassettevector for other uses pMB1 ori - rop cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR30 [WM2190, pNON11]
Depositors W Messer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.1659998893737790
Vector: pLDR30 (plasmid)
Construction: pT7-7
Marker(s):ampR
Construct size (kb): 2.165999889373779
Features: marker(s): ampR
plasmid replicon cassette: pMB1 ori - rop cassette
MCS: ClaI...EcoRI
Applications
contains easily purifiable cassette(s) for construction pMB1 ori - rop cassette
vector for other uses pMB1 ori - rop cassette
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.15; EcoRI--2.15; NotI--1.4, 0.85.
Helper plasmid with a NotI cassette containing a replication origin from pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map