Size (kb): 5.4739999771118160
Vector: p414 GAL1 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pRS414, GAL1 promoter
Construct size (kb): 5.473999977111816
Features: marker(s): TRP1
promoter for expression: GAL1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3.
Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose.
Low copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736