YCp50-poly (ATCC® 87555)

Applications: YC-type (centromeric) shuttle vectorvector for constructing genomic libraries  /  Depositors: DJ Stillman

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Designations YCp50-poly
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 7.0560002326965330
Vector: YCp50-poly (plasmid)
Construction: YCp50, pUC19 polylinker
Marker(s):URA3,ampR
Construct size (kb): 7.056000232696533
Features: marker(s): URA3
marker(s): ampR
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
vector for constructing genomic libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; SmaI--5.1, 2.2; PstI--3.3, 1.8, 1.1, 0.9; PvuI--7.2.
YC-type shuttle vector.
YCp50-poly was constructed in two steps: (1) YCp50 (ATCC 37419) was cleaved with XhoI and Asp718, blunted and ligated. (2) This construct was then cleaved with EcoRI and SalI, and the EcoRI-PvuII fragment of pUC19 was inserted.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414

Related Products
component of:ATCC 87635
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.

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Attachments Vector map for 87555