YCplac33 (ATCC® 87586)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

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Designations YCplac33
Depositors RD Gietz
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6030001640319820
DESCRIPTION OF VECTOR:
Intact vector size: 5.603
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Other unique sites: EcoRV NdeI AatII NarI SpeI NaeI NheI BglII ClaI NsiI StuI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): URA3, <-, 2563-3365
marker(s): ampR, ->, 3802-4662
replicon: pMB1, 5420-5420
Vector: YCplac33 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):URA3,ampR
Construct size (kb): 5.603000164031982
Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--3.7, 1.9; ClaI--5.7; PstI--5.6.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

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Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87586