Blastocrithidia culicis (Novy et al.) Wallace and Johnson (ATCC® 30268)

Organism: Blastocrithidia culicis (Novy et al.) Wallace and Johnson  /  Depositor: KP Chang

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Biosafety Level 1
Isolation
mosquito, Aedes vexans, 1942
Product Format frozen
Type Strain no
Comments
Endosymbiont-bearing
Nutrition significance of symbiotic bacteria
monophyly of endosymbiont-containing trypanosomatids
Effects of methylglyoxal bis (guanylhydrazone) on growth
Ultrastructure of symbiotic bacteria
RNA editing
Cell-to-cell interactions
Trypomastigotes in cultures
Cyclopropane fatty acid
Medium Medium 832: Grace’s insect medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor KP Chang
Chain of Custody
ATCC <<--KP Chang<<--F.G. Wallace
Year of Origin 1942
References

Chang KP. Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti. J. Protozool. 21: 699-707, 1974. PubMed: 4217371

Chang KP, et al. Effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149, 1978. PubMed: 660567

Chang KP, Trager W. Nutritional significance of symbiotic bacteria in two species of hemoflagellates. Science 183: 531-532, 1974. PubMed: 4203488

Maslov DA, et al. Evolution of RNA editing in kinetoplastid protozoa: pan-editing preceded 5'-editing. Nature 368: 345-348, 1994. PubMed: 8127370

Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247

Sousa MA. Cell-to-cell interactions suggesting a sexual process in Herpetomonas megaseliae (Kinetoplastida: Trypanosomatidae). Parasitol. Res. 80: 112-116, 1994. PubMed: 8202449

de Sousa MA. Trypomastigotes in cultures of Blastocrithidia culicis (Novy, MacNeal & Torrey, 1907) (Kinetoplastida: Trypanosomatidae). Mem. Inst. Oswaldo Cruz Rio J. 89: 111-112, 1994. PubMed: 7823806

Hollar J, et al. Monophyly of endosymbiont containing trypanosomatids: Phylogeny versus taxonomy. J. Eukaryot. Microbiol. 45: 293-297, 1998. PubMed: 9627990

Du Y, et al. Monophyletic origin of beta-division proteobacterial endosymbionts and their coevolution with insect trypanosomatid protozoa. Proc. Natl. Acad. Sci. USA 91: 8437-8441, 1994. PubMed: 7521530

Cross References

Nucleotide (GenBank) : L29266 ribosomal RNA sequence

Nucleotide (GenBank) : L29265 Blastocrithidia culicis ribosomal RNA.

Nucleotide (GenBank) : U05817 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial pre-RNA-edited DNA sequence

Nucleotide (GenBank) : U05813 kinetoplast (MURF1) and apocytochrome B (cytB) genes, partial coding sequence, and ATPase subunit 6 (MURF4) gene, complete pre-RNA-edited DNA sequence

Nucleotide (GenBank) : U05814 kinetoplast NADH dehydrogenase subunit 7 (ND7) gene, partial coding sequence, and cytochrome c oxidase subunit III (COIII) gene, complete coding sequence

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation