Acanthamoeba castellanii (Douglas) Page (ATCC® PRA-108)

Strain Designations: 9GU  /  Depositor: J Walochnik  /  Biosafety Level: 2

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Strain Designations 9GU
Biosafety Level 2
Isolation
clinical specimen - human
Graz Austria
Isolation date: 1996
Product Format frozen
Storage Conditions liquid nitrogen vapor phase
Type Strain no
Medium Medium 712: PYG w/ Additives
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
Interval: every month
Subcultivation
Protocol: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the shipment arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. After thawing the ampule, transfer the contents to a 16 x 125 mm plastic screw-capped test tube containing 5 ml of fresh medium. (Glass test tubes may also be used, but the cultures can be transferred less frequently when maintained in plastic.) Screw the cap on tightly and incubate the tube on a 5-15 degree slant at the appropriate temperature. Subculture every 2-4 weeks by vigorously agitating the culture and aseptically transferring a 0.1 ml aliquot to a fresh tube of medium. Prolongation of the transfer interval can be extended up to 6 months for certain strains of Acanthamoeba, however, this must be determined empirically for each strain.
Interval: every month
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Name of Depositor J Walochnik
Chain of Custody
J Walochnik
Year of Origin 1996
References

Walochnik J, et al. Discrimination between clinically relevant and nonrelevant Acanthamoeba strains isolated from contact lens- wearing keratitis patients in Austria. J. Clin. Microbiol. 38: 3932-3936, 2000. PubMed: 11060047

Walochnik J, et al. Correlations between morphological, molecular biological, and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp. Appl. Environ. Microbiol. 66: 4408-4413, 2000. PubMed: 11010891

Walochnik J, et al. Immunological inter-strain crossreactivity correlated to 18S rDNA sequence types in Acanthamoeba spp. Int. J. Parasitol. 31: 163-167, 2001. PubMed: 11239936

isolated from the contact lens case of an asymptomatic patient

Cross References

Nucleotide (GenBank) : U07413 Acanthamoeba castellanii Castellani ATCC 50374 18S rRNA gene

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