FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
This strain is a tissue culture-adapted version of strain TW-3 (VR-578) which is grown in embryonated chicken eggs.
Before infecting host cells, add 1-mm glass beads and vortex stock material for 45 seconds. Aspirate fluid. Infect shell vial monolayer with 0.2 mL of material.
Centrifuge at 800 x G for 1 hour. Feed with 1.0 mL EMEM (HEPES buffered), 10% FBS (screened for chlamydia antibodies).
Effect on Host
Requires FA to visualize intracellular inclusion bodies