Chlamydia trachomatis Serovar I (ATCC® VR-880)

Classification: Chlamydiacae  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Chlamydiacae
Agent Chlamydia trachomatis Serovar I
Strain UW-12/Ur
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Add glassbeads and vortex preparation to disrupt cells.  Infect monolayer with disrupted material.  Centrifuge at 3000 x rpm (750 x g) for 1 hour.  Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.  Incubate at 37°C for 48 hours.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 1500 x g for 1 hour at 25°C. End adsorption by adding growth medium.
Incubation: 1-3 days
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Name of Depositor SP Wang
Source Male urethra with non-gonococcal urethritis
References

Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation