Chlamydia trachomatis Serovar I (ATCC® VR-880)

Classification: Chlamydiacae  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Chlamydiacae
Deposited As Trachoma serotype i
Agent Chlamydia trachomatis Serovar I
Strain UW-12/Ur
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

Note that activities with high potential for aerosol production require BSL 3 facilities and practices.

Add glassbeads and vortex preparation to disrupt cells.  Infect monolayer with disrupted material.  Centrifuge at 3000 x rpm (750 x g) for 1 hour.  Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.  Incubate at 37°C for 48 hours.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 1500 x g for 1 hour at 25°C. End adsorption by adding growth medium.
Incubation: 1-3 days
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Name of Depositor SP Wang
Source Male urethra with non-gonococcal urethritis
References

Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation