Chlamydia trachomatis (ATCC® VR-887)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen

Permits and Restrictions

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Classification Chlamydiaceae, Chlamydia
Deposited As trachoma serotype k
Agent Chlamydia trachomatis
Strain Trachoma type K strain UW-31/Cx
Applications
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Biosafety Level 2
Isolation
human cervix, cervicitis, Seattle, WA
Product Format frozen
Storage Conditions -70.0°C or colder
Comments
Closely related immunologically with LGV Type L III.
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37°C for 48 hours.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Recommended Host
McCoy cells (ATCC CRL-1696)
HeLa 229 cells (ATCC CCL-2.1)
Growth Conditions
Temperature: 36.5°C
Incubation: 2 days at 37°C
Cryopreservation
Storage temperature: -70°C or lower
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Name of Depositor SP Wang
Source
human cervix, cervicitis, Seattle, WA
References

Kuo CC, et al. TRIC type K, a new immunologic type of Chlamydia trachomatis. J. Immunol. 113: 591-596, 1974. PubMed: 4210884

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation