West Nile virus (ATCC® VR-1612)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen Each vial of VR-1612 contains approximately 100 µL of purified monoclonal antibody in PBS.

Classification Flaviviridae, Flavivirus
Agent West Nile virus
Strain Monoclonal antibody to West Nile virus Envelope Protein, Clone E24 (produced in vitro)
Common Name West Nile virus
Applications
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography.
The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis.
Emerging infectious disease research
Biosafety Level 1
Isolation
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.
Product Format frozen Each vial of VR-1612 contains approximately 100 µL of purified monoclonal antibody in PBS.
Storage Conditions  Store at -20°C or colder immediately upon arrival Repeated freeze-thaw cycles should be avoided
Comments
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.
Functional activity is confirmed by reactivity in immunofluorescence assays using Vero cells (ATCC CCL-81) infected with WNV and by ELISA using WNV-infected cell lysates [WNV, Eg101 (Egypt 1951)]. The antibody is reported to be strongly neutralizing and reactive using flow cytometry and Western blot analysis.
Effect on Host
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.
Effect on Host
Mouse monoclonal antibody prepared against the envelope glycoprotein of West Nile virus (WNV) was purified from clone E24 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63.Ag8.53 BALB/c mouse myeloma cells with immunized mouse splenocytes. The clone E24 antibody is reported to bind to the lateral ridge of domain III in the envelope glycoprotein.
Name of Depositor MS Diamond
References

Oliphant T, et al. Development of a Humanized Monoclonal Antibody with Therapeutic Potential Against West Nile Virus. Nat. Med. 11: 522-530, 2005. PubMed:15852016.

Oliphant T, et al. Antibody Recognition and Neutralization Determinants on Domains I and II of West Nile Virus Envelope Protein. J. Virol. 80:12149-12159, 2006. PubMed:17035317.