Chlamydophila pneumoniae (Grayston et al.) Everett et al. (ATCC® VR-1435)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae Grayston et al.
Agent Chlamydophila pneumoniae (Grayston et al.) Everett et al.
Strain J-21
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Isolate from patient in Japan

This preparation has tested positive for Mycoplasma contamination

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Recommended Host
HEp-2 cells (ATCC CCL-23)
Growth Conditions Temperature: 35°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Name of Depositor N Tsumura
Source Throat culture of 12-year-old boy in Japan with pneumonia
References

Tsumura N. Chlamydia pneumoniae lower respiratory tract infections in Japanese children. Abstract: Annual Meeting ICAAC. San Francisco: ; 1995.