Waddlia chondrophila Everett et al. (ATCC® VR-1470)

Classification: Waddliaceae  /  Product Format: frozen

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Classification Waddliaceae
Agent Waddlia chondrophila Everett et al.
Strain WSU 86-1044
Applications
Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp.
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
Biosafety Level 2
Isolation
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
Product Format frozen
Storage Conditions -70°C or colder
Genome Sequenced Strain

Yes

Comments
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. and 14 members of the genus Rickettsia all failed to react significantly. Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate).
Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496.
MEM with 10% serum
Effect on Host
Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell
Inclusions visualized by light microscopy as dense forms.  Some cell death due to lysis.  Cells become rounded and dense.
Recommended Host
bovine turbinate (BT) cells (ATCC CRL-1390)
BT cells (ATCC CRL-1390); P388D1 mouse macrophage cells (ATCC CCL-46)
Growth Conditions
Incubation: 3 days at 35°C in BT cells (ATCC CRL-1390), 5% CO2
Effect on Host
Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell
Inclusions visualized by light microscopy as dense forms.  Some cell death due to lysis.  Cells become rounded and dense.
Name of Depositor TF McElwain
Special Collection NCRR Contract
Source
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation
References

Dilbeck PM, et al. Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J. Clin. Microbiol. 28: 814-816, 1990. PubMed: 2185269

Kocan KM, et al. Development of a rickettsia isolated from an aborted bovine fetus. J. Bacteriol. 172: 5949-5955, 1990. PubMed: 2211519

Rurangirwa FR, et al. Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 49: 577-581, 1999. PubMed: 10319478

type strain

Terry F McElwain, personal communication

Cross References

Nucleotide (GenBank) : AF042496 16S ribosomal RNA and 23S ribosomal RNA, complete sequence

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation