Chlamydia suis Everett et al. (ATCC® VR-1474)

Classification: Chlamydiaceae  /  Product Format: frozen

Classification Chlamydiaceae
Agent Chlamydia suis Everett et al.
Strain S45 (porcine isolate)
Biosafety Level 2
Product Format frozen
Storage Conditions Vapor phase of liquid nitrogen
Comments Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.  Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).  Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.  End adsorption by adding agent growth medium.
Incubation:  Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere.
Preservation: Perform slow controlled rate freeze.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor J Storz
Special Collection NCRR Contract
Source Swine intestine, feces, and diarrhea fluid
References

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

Kaltenboeck B, et al. Structures of and allelic diversity and relationships among the major outer membrane protein (ompA) genes of the four chlamydial species. J. Bacteriol. 175: 487-502, 1993. PubMed: 8419295

type strain

Cross References

Nucleotide (GenBank) : AJ012176 Chlamydia suis partial rnpB gene for RNase P RNA subunit.

Nucleotide (GenBank) : AF269274 Chlamydia suis S45 major outer membrane protein (ompA) gene, partial cds; and tRNA-Gly gene, partial sequence.