Chlamydophila abortus (ATCC® VR-656)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen

Classification Chlamydiaceae, Chlamydophila
Deposited As Ovine chlamydial abortion
Agent Chlamydophila abortus
Strain B-577
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Kidney from aborted ovine fetus
Product Format frozen
Storage Conditions Vapor phase of liquid nitrogen
Images Chlamydophila abortus ATCC VR-656 images
Comments Please see ATCC Virology Guide for more detailed information on propagation
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer. Sonicate seed material for 20 seconds at approximately 240W to disrupt cells.  Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2).  Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour.  End adsorption by adding agent growth medium.
Incubation:  Incubate infected culture for 72 hours at 37°C in a humidified 5% CO2 atmosphere.
Preservation: Controlled-rate freezing.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining
Name of Depositor J Storz
Source Kidney from aborted ovine fetus
References

Storz J. Psittacosis-lymphogranuloma infection of sheep. Antigenic structures and interrelations of PL agents associated with polyarthritis, enzootic abortion, intrauterine and latent intestinal infections. J. Comp. Pathol. 76: 351-362, 1966. PubMed: 6008377

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

type strain

Cross References

Nucleotide (GenBank) : D85709 Chlamydophila abortus DNA for 16S ribosomal RNA.

Nucleotide (GenBank) : AB001783 Chlamydophila abortus gene for 16S rRNA, strain B577.

Nucleotide (GenBank) : AJ012170 Chlamydophila abortus partial rnpB gene for RNase P RNA subunit.

Nucleotide (GenBank) : U68445 Chlamydophila abortus B577 16S ribosomal RNA gene, partial sequence, intergenic spacer, and 23S ribosomal RNA gene, partial sequence.