Chlamydia trachomatis (ATCC® VR-903)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen

Classification Chlamydiaceae, Chlamydia
Deposited As Lymphogranuloma venereum type III
Agent Chlamydia trachomatis
Strain Lymphogranuloma venereum (LGV III) strain 404
Applications
Sexually Transmitted Disease Research
Biosafety Level 3
Isolation
Lymph node from human with LGV
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Effect on Host
Cytopathic effects, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen round middle. Inclusions best visualized by fluorescent antibody.
CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA.  Death of chicken embryo.
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate and supplemented with 2 mcg/ml Cycloheximide, 10 mM HEPES and 10% fetal bovine serum (prescreened for chlamydia antibodies)
Recommended Host
McCoy (ATCC CRL-1696)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37°C
Recommendations for Infection: 2-3 days; For best results in tissue culture, cells should be 24-48 hours old and 80-100% confluent. The cells will not continue to grow after adding cycloheximide.
Cryopreservation
Storage temperature: -70°C
Effect on Host
Cytopathic effects, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen round middle. Inclusions best visualized by fluorescent antibody.
CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA.  Death of chicken embryo.
Name of Depositor J Schachter
Source
Lymph node from human with LGV
References

Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618

Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529

or colder

In addition to the above, this virus can be grown in a variety of animal cell lines.

or colder