Influenza A virus (H1N1) (ATCC® VR-1520)

Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen 1 mL per vial

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Classification Orthomyxoviridae, Influenzavirus A
Deposited As Influenza A virus
Agent Influenza A virus (H1N1)
Strain A/WS/33
Applications
Influenza Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1520
Comments
Derived by adaptation of egg allantoic fluid product ATCC® VR-825™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-825™ and ATCC® VR-1520™ have not been compared for sequence or infectivity in eggs and tissue culture. Demonstrates cross-reactivity with Influenza A virus, A/PR/8/34 and swine influenza.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
MDCK (ATCC® CCL-34™)
Growth Conditions
Temperature: 33°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor WA Chappell, Centers for Disease Control and Prevention; ATCC
Special Collection NCRR Contract
Source Throat washings from an influenza patient
References

Burnet FM. A genetic approach to variation in influenza viruses; the characters of three substrains of influenza virus A (WS). J. Gen. Microbiol. 5: 46-53, 1951. PubMed: 14824469

Smith W, et al. A virus isolated from influenza patients. Lancet 2: 66-68, 1933.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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