Recommendations for Infection: Plate cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI 0.1. Adsorb 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Grow in serum free media that contains 2.0μg/ml TPCK-treated trypsin.
Incubation: 2-5 days