Avian coronavirus (ATCC® VR-22)

Classification: Coronaviridae, Gammacoronavirus  /  Product Format: frozen

Classification Coronaviridae, Gammacoronavirus
Deposited As Infectious bronchitis virus
Agent Avian coronavirus
Strain Egg-adapted (Michigan State Univ. Repos. Code 42)
Common Name Infectious bronchitis virus (IBV)
Applications
For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity.
Biosafety Level 2
Isolation
Specimen from chicks with naturally occurring disease, New Jersey, 1935
Product Format frozen
Storage Conditions -70°C or colder
Comments
Reported to cause syncytial CPE in CEK TC with subsequent necrosis of cells.
For passage in eggs, clarify sample and use supenatant fluid for inoculum via allantoic cavity. Chill eggs for harvest when one or two enbryos are dead and the remainder are week or sluggish.
Effect on Host
Yes, in vivo effects: death of chicken embryo
Death of CE
Yes, in vitro effects: syncytia in chicken embryo kidney tissue culture
Recommended Host
10 day chicken embryo, i.a. inoculation

Host of Choice: CE (10 day, i.a.)

Host Range: CE, CAM and CEK TC

chicken embryo (chorioallantoic membrane); chicken embryonic kidney cells in tissue culture
Growth Conditions
Duration: 1-2 days at 37C, via allantoic route
Effect on Host
Yes, in vivo effects: death of chicken embryo
Death of CE
Yes, in vitro effects: syncytia in chicken embryo kidney tissue culture
Name of Depositor CH Cunningham
Special Collection ATCC
Source
Specimen from chicks with naturally occurring disease, New Jersey, 1935
Year of Origin 1935
References

Cunningham CH. . Adv. Vet. Sci. 14: 106-148, 1970.

McIntosh K. . Curr. Top. Microbiol. Immunol. 63: 86-119, 1974.

Beaudette FR. . J. Am. Vet. Med. Assoc. 90: 51, 1937.