Murine hepatitis virus (ATCC® VR-766)

Classification: Nidovirales, Coronaviridae, Coronavirus,Group 2  /  Product Format: frozen

Classification Nidovirales, Coronaviridae, Coronavirus,Group 2
Agent Murine hepatitis virus
Strain MHV-S Log #3239/17
Common Name Murine hepatitis virus
Biosafety Level 2
Isolation
Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice
Product Format frozen
Storage Conditions -70°C or colder
Comments
Highly infectious in susceptible mouse colonies.
Despite high mortality, the clinical signs in infected mice are often non-specific, with weakness or are suggestive of encephalitis with spasticity. Necropsy occasionally reveals small yellow or whitish spots on the liver.
Highly infectious in susceptible mouse colonies.
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, apoptosis and slough off in clumps
Recommended Host
NCTC clone 1469 cells (ATCC CCL-9.1)

Recommended Host: NCTC clone 1469 cells (ATCC® CCL-9.1™)

newborn mouse; NCTC clone 1469 cells (ATCC CCL-9.1)
Growth Conditions
Temperature: 37°C
Incubation: 18 to 48 hours at 37°C, a 5% CO2 in air atmosphere is recommended
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE, apoptosis and slough off in clumps
Name of Depositor MJ Collins
Special Collection ATCC
Source
Isolated by i.c. and i.p. passage of viscera of 8 sick CD-1 mice in infant and suckling mice
References

Hartley JW, et al. Mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection of mice. Proc. Soc. Exp. Biol. Med. 112: 161-165, 1963. PubMed: 13982823