Quantitative Synthetic West Nile Virus RNA (ATCC® VR-3198SD)

Product Format: frozen powder
Specification range: >1.0 x 108 copies

Permits and Restrictions

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Agent Quantitative Synthetic West Nile Virus RNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen powder
Specification range: >1.0 x 108 copies
Storage Conditions 8°C or colder

Genetic Target: Preparation includes fragments from the 5'UTR region, anchored capsid protein C gene, capsid protein C gene, membrane glycoprotein precursor prM gene, envelope protein E gene, nonstructural protein NS1, NS2A, NS3, and NS5 genes, and the 3' UTR region of the West Nile Virus genome. The length of the nucleic acid is approximately 1500 bp. 

Length: ~1500 bp

Format Provided: Dried RNA  

Starting Amount: 1.0 x 108 Copy Numbers (CN)  

Downstream compatibility: Reverse Transcription PCR or other molecular applications  

Special Collection DNA

Briese T, Glass W, Lipkin W. Detection of West Nile virus sequences in cerebrospinal fluid. Lancet 355(9215): 1614-1615, 2000 PubMed: 10821368

Eiden M, et al. Two new real-time quantitative reverse transcription polymerase chain reaction assays with unique target sites for the specific and sensitive detection of lineages 1 and 2 West Nile virus strains. J Vet Diagn Invest 22(5): 748-753, 2010 PubMed: 20807934

Jimenez-Clavero M, et al. A new fluorogenic real-time RT-PCR assay for detection of lineage 1 and lineage 2 West Nile viruses. J Vet Diagn Invest 18(5): 459-462, 2006 PubMed: 17037613

Lanciotti R, et al. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitos, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 38(11): 4066-4071, 2000 PubMed: 11060069

Papin J, Vahrson W, Dittmer D. SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability. J Clin Microbiol 42(4): 1511-1518, 2004 PubMed: 15070997

Parida M, et al. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol 42(1): 257-263, 2004 PubMed: 14715762

Shi P, et al. High-throughput detection of West Nile virus RNA. J Clin Microbiol 39(4): 1264-1271, 2001 PubMed: 11283039

Usuku S, Noguchi Y, Takasaki T. Newly developed TaqMan assay to detect West Nile viruses in a wide range of viral strains. Jpn J Infect Dis 57(3): 129-130, 2004 PubMed: 15218227

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Stability of Norovirus and West Nile standards

    Accelerated stability studies at 45oC have been performed on these samples and no degradation was observed.

    Date Updated: 8/11/2015