DNA from Chlamydia trachomatis strain UW-36/Cx (ATCC® VR-886D)

Organism: Chlamydia trachomatis Serovar J  / 

Product Format frozen >1 µg/mL in 1X TE buffer, 100 µL per vial
Storage Conditions Store at -70°C or colder
Shipping Information Frozen
Applications
DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Sexually Transmitted Disease Research
Comments
Chlamydia DNA, in total DNA isolated from HeLa-229 cells (ATCC CCL-2.1) infected with Chlamydia trachomatis strain UW-36/Cx (ATCC VR-886). Organism and host cells are also available through the ATCC Catalog. This product was prepared using methods known to inactivate the infecting agent. DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Organism and host cells are also available through the ATCC Catalog.
Focus Area
Infectious diseases
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as > 1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (> 1.7) of bulk DNA prior to dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 ul of product on a 0.8% agarose gel, visualized by ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 600-12,000 bp amplicon using Chlamydia-specific primers.

6. Identity is confirmed by sequencing of ~ 1Kb PCR amplicon.