Media Sera Reagents

  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Phosphate Buffered Saline Without Calcium Or Magnesium (ATCC® SCRR-2201)

    ATCC® Number: SCRR-2201
    Quantity: 500 mL
    Storage: Store at ambient temperatures, between 15°C and 30°C.
    Applications:

    For washing cells (including embryonic stem cells), for use as a diluent, or for use as an inorganic base for standard media preparation.

    For-Profit:   $13.00 Non-Profit:   $13.00

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  • Non-Enzymatic Cell Dissociation Solution (ATCC® 30-2103)

    ATCC® Number: 30-2103
    Quantity: 100 mL
    Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
    Applications:

    Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. 

    For-Profit:   $27.00 Non-Profit:   $27.00

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    Product Format: 100 mL
    Comments: This product is totally free of animal-derived components.
  • Vascular Cell Basal Medium (ATCC® PCS-100-030)

    ATCC® Number: PCS-100-030
    Quantity: 475 mL
    Storage: 2°C to 8°C, protect from light
    Applications: Vascular Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system.

    This system is designed to support cells derived from normal human large vessels such as human umbilical vein endothelial cells, aortic endothelial cells or aortic smooth muscle cells, as well as microvascular endothelial cells. Vascular Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of cells derived from the vascular system, Vascular Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit.

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  • TSB with 10% Glycerol (ATCC® 20-2200)

    ATCC® Number: 20-2200
    Quantity: Package of 12 (1.0 mL/ vial)
    Storage: Store at 4 to 8°C
    Applications:

    Trypticase soy broth (TSB) with 10% glycerol (v/v) is used as a freeze medium for storage of nonfastidious bacteria, fungi, and yeast. Product is provided in an individual unit of use (1.0 mL).

    For-Profit:   $65.00 Non-Profit:   $65.00

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  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service.  ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines.
  • Renal Epithelial Cell Basal Medium (ATCC® PCS-400-030)

    ATCC® Number: PCS-400-030
    Quantity: 485 mL
    Storage: 2 to 8°C, protect from light
    Applications: Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of renal epithelial cells (e.g., Primary Renal Proximal Tubule Epithelial Cells, Normal, Human, ATCC PCS-400-010; Primary Renal Cortical Epithelial Cells, Normal, Human, ATCC PCS-400-011; Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040).

    Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates.

    Optional media supplements:

    1. Gentamicin-Amphotericin B Solution  (ATCC PCS-999-025)
    2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    3. Phenol Red (ATCC PCS-999-001)

    For-Profit:   $50.00 Non-Profit:   $50.00

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  • HEKPlus SFM (ATCC® ACS-4002)

    ATCC® Number: ACS-4002
    Quantity: 1 L
    Storage: Store at 2°C to 8°C. Do not freeze. Protect from light.

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    Product Format: 1 L
    Comments:
    HEKPlus SFM should be supplemented with L-alanyl-glutamine or L-glutamine prior to use. The medium contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus SFM also includes human transferrin and recombinant human insulin that have been shown to improve cell proliferation.

    This medium has been optimized for use with ATCC HEK293T/17 SF suspension cells (ATCC ACS-4500) and GeneX Plus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels. HEK293T/17 SF cells are pre-adapted to the medium and can be cultured in suspension and directly transfected in HEKPlus SFM using GeneXPlus transfection reagent. Transfection complexes can be prepared in the HEKPlus medium as well. In general, HEK293 cells currently adapted to serum-free medium can be subcultured directly using higher seeding densities. For cells currently adapted to serum containing medium, sequential adaptation to HEKPlus SFM is recommended.