Media Sera Reagents

  • McCoy's 5A Medium (ATCC® 30-2007)

    ATCC® Number: 30-2007
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.


  • Horse Serum (ATCC® 30-2041)

    ATCC® Number: 30-2041
    Quantity: 100 mL
    Storage: Store horse serum at -20°C or colder. Do not store frozen horse serum at temperatures above -20°C for any length of time. Avoid repeated freeze-thaws by dispensing and storing horse serum in aliquots. Horse serum is supplied in PETG plastic bottles.
    Applications: Triple filtered through 0.1 μm filters. Each lot of horse serum is tested for sterility and for the ability to support the growth of cells using sequential growth curves. Horse serum is manufactured from blood collected from select donor horse herds maintained within the United States.

    For-Profit:   $19.00 Non-Profit:   $19.00

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  • L-Glutamine Solution, 200 mM (ATCC® 30-2214)

    ATCC® Number: 30-2214
    Quantity: 100 mL
    Storage: Store L-glutamine solution frozen at -20°C or colder. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications: L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture.

    L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH.

    All cell culture media are formulated to contain appropriate amounts of L-glutamine to support cell growth well beyond the expiration date of the media. However, if L-glutamine is suspected to be a limiting factor during cell culture, a simple test of 'spiking' the medium with a small amount of L-glutamine will determine whether or not more is required. Simply add a small amount of L-glutamine (~ 2 mM final concentration) to the culture medium. If the cell growth rate increases, L-glutamine is most likely deficient and more should be added.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Product Format: frozen 100 mL
    Comments:

    Added as a supplement to cell culture media. L-Glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. L-Glutamine is also more labile in cell culture solutions than other amino acids. The rate and extent of L-glutamine degradation are related to storage temperatures, age of the product, and pH. 

    Note: L-glutamine degradation results in the build-up of ammonia which can have a deleterious effect on some cell lines. Use caution when adding more L-glutamine than called for in the original medium formulation (2 to 4 mM is standard). For most cell lines, ammonia toxicity is more critical for cell viability than L-glutamine limitation.

    For more information, please contact ATCC Technical Service.

  • Dulbecco's Phosphate Buffered Saline (D-PBS), 1X (ATCC® 30-2200)

    ATCC® Number: 30-2200
    Quantity: 500 mL
    Storage: Store Dulbecco's PBS at ambient temperatures, between 15°C and 30°C.
    Applications:

    To be used for washing cells,  as a diluent, or as an inorganic base for standard media preparation.

    For-Profit:   $15.00 Non-Profit:   $15.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    Formulation contains no calcium or magnesium.

  • Penicillin-Streptomycin Solution (ATCC® 30-2300)

    ATCC® Number: 30-2300
    Quantity: 100 mL
    Storage: -20°C or colder
    Applications:

    10,000 I.U./mL Penicillin, 10,000 (μg/mL) Streptomycin For reducing the chances of microbial contamination in cell culture. Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 (μg/mL) streptomycin.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Product Format: frozen 100 mL
    Comments: NOTE: Most cell culture technologists avoid using antibiotics and antimycotics for routine culture work. Antibiotics and antimycotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics and antimycotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics and antimycotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks.
  • Vascular Cell Basal Medium (ATCC® PCS-100-030)

    ATCC® Number: PCS-100-030
    Quantity: 475 mL
    Storage: 2°C to 8°C, protect from light
    Applications: Vascular Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system.

    This system is designed to support cells derived from normal human large vessels such as human umbilical vein endothelial cells, aortic endothelial cells or aortic smooth muscle cells, as well as microvascular endothelial cells. Vascular Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. To support the proliferation and plating efficiency of cells derived from the vascular system, Vascular Cell Basal Medium must be supplemented with the appropriate cell-specific growth kit.

    For-Profit:   $50.00 Non-Profit:   $50.00

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  • Dimethylsulfoxide (DMSO) (ATCC® 4-X)

    ATCC® Number: 4-X
    Quantity: 5 x 5 mL vials
    Storage: Store DMSO at 2°C to 8°C when not in use. It may be solid at this temperature. However, it may also be stored at room temperature where it remains a liquid.
    Applications:

    DMSO is used as a cyroprotectant for the cryopreservation of cell lines. ATCC DMSO is cell culture grade and has been tested to assure nontoxicity and sterility.

    For-Profit:   $47.00 Non-Profit:   $47.00

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  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.