Penicillin-Streptomycin Solution (ATCC® 30-2300)

Product Format frozen 100 mL
Storage Conditions -20°C or colder
Applications
10,000 I.U./mL Penicillin, 10,000 (μg/mL) Streptomycin For reducing the chances of microbial contamination in cell culture. Between 0.5 and 1 mL of Penicillin-Streptomycin solution are added to 100 mL of cell culture media for a final concentration of 50 to 100 I.U./mL penicillin and 50 to 100 (μg/mL) streptomycin.
Comments NOTE: Most cell culture technologists avoid using antibiotics and antimycotics for routine culture work. Antibiotics and antimycotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics and antimycotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics and antimycotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks.
Biosafety Level 1
Quality Control Specifications

Lot-specific test results are listed in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC Technical Service.

SPECIFICATION CERTIFICATE

Test

Specification

pH1

Report results

Osmolality2

Report results

Endotoxin3

≤ 0.25 EU/mL @ 1:100

Antimicrobial Activity4 (1:100)

Inhibition of S. aureus & E. coli

Sterility5

Pass

  1. Measured with a standard pH meter.  Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.
  2. Measured with a standardized osmometer, i.e. freezing point depression.  Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.
  3. Chromogenic LAL assay using guidelines outlined by the manufacturer & FDA (December 1987), “Validation of the Limulus Amebocyte Lysate Test as an End Product Endotoxin Test for Human and Animal Parenteral Drugs, Biological Products, and Medical Devices”.
  4. Measured using Minimal Inhibitory Concentration (MIC).  At 100 CFU/mL, MIC must meet user concentration.
  5. Tested by a modified membrane filtration technique.