Dissociation Reagents

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  • Soybean Trypsin Inhibitor (ATCC® 30-2104)

    ATCC® Number: 30-2104
    Quantity: 20 mL (5 mg/mL)
    Storage: When stored at -20°C, the product is stable until the expiration date on the label.
    Applications:

    The use of Soybean Trypsin Inhibitor (SBTI) provides a serum-free option with which to inactivate trypsin when subculturing adherent cells.

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    Product Format: frozen 20 mL (5 mg/mL)
  • Non-Enzymatic Cell Dissociation Solution (ATCC® 30-2103)

    ATCC® Number: 30-2103
    Quantity: 100 mL
    Storage: When stored at 2°C to 8°C, the product is stable until the expiration date on the label.
    Applications:

    Trypsin is the most commonly used enzyme for harvesting cells in culture. A non-enzymatic approach is needed when non-protein and animal-component free materials are required. ATCC Non-Enzymatic Cell Dissociation Solution is a sterile, phenol-red free solution composed of a proprietary mixture of chelators representing an optimized alternative to protein-digesting enzymes. 

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    Product Format: 100 mL
    Comments: This product is totally free of animal-derived components.
  • Trypsin-EDTA Solution, 1X (ATCC® 30-2101)

    ATCC® Number: 30-2101
    Quantity: 100 mL
    Storage: Store Trypsin-EDTA solution frozen, between -5°C and -20°C. Avoid repeated freeze-thaws by dispensing and storing in aliquots.
    Applications:

    0.25% Trypsin/0.53 mM EDTA in Hanks Balanced Salt Solution without calcium or magnesium. For dissociation of cell monolayers. Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. For cell line-specific information, please go to the appropriate product page on the web, refer to the product sheet supplied with the cell line, or contact ATCC Technical Service.

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    Product Format: frozen 100 mL
    Comments: NOTE:  Each cell line responds to ATCC® Trypsin-EDTA solution in a unique manner.  For optimum results, observe the cells during the dissociation process to avoid over-trypsinization.  For more information, please refer to the General Protocol for Using ATCC® Trypsin-EDTA Solution.  For cell line-specific information, please refer to the product sheet supplied with the cell line, or contact ATCC® Technical Service. ATCC® Trypsin-EDTA solution is suitable for most but not for all adherent cell lines. This product contains phenol red.
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