YB2/0 [YB2/3HL.P2.G11.16Ag.20] (ATCC® CRL-1662)

Organism: Rattus norvegicus, rat  /  Cell Type: hybridoma: B lymphoblast  /  Tissue: spleen  / 

Organism Rattus norvegicus, rat
Tissue spleen
Cell Type hybridoma: B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Derivation
This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.
Comments
Tested and found negative for ectromelia virus (mousepox).
The cells do not secrete either heavy or light immunoglobulin chains.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/mL and maintain at or below 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium 95%; DMSO 5% (v/v). Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Name of Depositor C Milstein
References

Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596

. . Methods Enzymol. 73B: 1-46, 1981.

Basic Documentation
References

Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596

. . Methods Enzymol. 73B: 1-46, 1981.