GK-5 (ATCC® CRL-1834)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed  / 

Organism Homo sapiens, human
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Applications
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Derivation
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
Genes Expressed
immunoglobulin; monoclonal antibody; against human kappa light chain
Cellular Products
immunoglobulin; monoclonal antibody; against human kappa light chain
Comments
GK-5 is a human lymphoblastoid cell line derived from the GM1500-GTG-A12 EBV transformed cell line.
The cells secrete very low levels (less than 10 ng/ml) of human kappa light chain.
The line has been used successfully to prepare human hybridomas.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol
  • fetal bovine serum to a final concentration of 10%

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor JF Kearney
References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036

Basic Documentation
References

Kearney JF. Hybridomas and monoclonal antibodies In: Kearney JF. Fundamental immunology: New YorkRaven Press pp. 751-766, 1984

Satoh J, et al. Human monoclonal autoantibodies that react with multiple endocrine organs. N. Engl. J. Med. 309: 217-220, 1983. PubMed: 6866036