WIL2-S (ATCC® CRL-8885)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: spleen  /  Disease: hereditary spherocytosis

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Organism Homo sapiens, human
Tissue spleen
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2  [Cells contain Epstein-Barr virus]

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease hereditary spherocytosis
Age 5 years
Gender male
Ethnicity Caucasian
This cell line is a suitable transfection host.
Storage Conditions liquid niotrogen vapor phase
Clinical Data
5 years
This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/mL.
Medium Renewal: Every 2 to 3 days
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
F13A01: 6,7
F13B: 10
FESFPS: 11,12
LPL: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Name of Depositor Lovelace Medical Foundation
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation