AKR1.G.1.OUAR.1.26 (ATCC® TIB-232™) Organism: Mus musculus, mouse / Disease: lymphoma General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Mus musculus, mouse Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease lymphoma Strain AKR/J Applications The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.The line may be useful for fusion studies and as a host for the transfection of cloned genes. Antigen Expression Thy-1; L3T4; Lyt-2; HSA Genes Expressed Thy-1; L3T4; Lyt-2; HSA Comments The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.The line may be useful for fusion studies and as a host for the transfection of cloned genes.The cells have the surface antigen properties of cortical thymocytes. Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Name of Depositor R Hyman References . . Immunogenetics 10: 261-271, 1980. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References . . Immunogenetics 10: 261-271, 1980.