Classic Cell Culture Media

  • AlphaMEM with Nucleosides without L-Glutamine. (ATCC® 30-2604)

    ATCC® Number: 30-2604
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    AlphaMEM (Alpha Minimum Essential Medium) is an enriched modification of MEM that is widely used in mammalian cell culture. This formulation is made in Earle’s salts and contains ribonucleosides and deoxyribonucleosides.

    For-Profit:   $31.00 Non-Profit:   $31.00

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    Product Format: 500 mL
    Comments: Supplement with 2 mM L-glutamine prior to use.
  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002)

    ATCC® Number: 30-2002
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003)

    ATCC® Number: 30-2003
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: Modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $19.00 Non-Profit:   $19.00

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    Product Format: 500 mL
    Comments: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004)

    ATCC® Number: 30-2004
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments: NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

    ATCC® Number: 30-2005
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Leibovitz's L-15 Medium (ATCC® 30-2008)

    ATCC® Number: 30-2008
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Leibovitz's L-15 Medium contains 2 mM L-glutamine and no sodium bicarbonate.

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This formulation is designed to be used without additional CO2 in the atmosphere of cell cultures.
  • McCoy's 5A Medium (ATCC® 30-2007)

    ATCC® Number: 30-2007
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.


  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.00 Non-Profit:   $25.00

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  • RPMI Medium without Phenol Red without L-Glutamine (ATCC® 30-2602)

    ATCC® Number: 30-2602
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    RPMI 1640 is a modification of McCoy’s 5A medium that contains a higher concentration of inositol. The medium is commonly used with mammalian myeloma and hybridoma cells. This formulation does not contain phenol red or L-glutamine.

    For-Profit:   $21.00 Non-Profit:   $21.00

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    Product Format: 500 mL
    Comments:

    Supplement with 2 mM L-glutamine prior to use. Modified to contain 10 mM HEPES, 1 mM sodium pyruvate, 4.5 g/L D-glucose, and 1.5 g/L sodium bicarbonate. Note: This reduced level of sodium bicarbonate is intended for use in a 5% COin air environment. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.