Classic Cell Culture Media

  • DMEM: F-12 Medium (ATCC® 30-2006)

    ATCC® Number: 30-2006
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    DMEM: F-12 Medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Dulbecco's Modified Eagle's Medium (DMEM) (ATCC® 30-2002)

    ATCC® Number: 30-2002
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Dulbecco's Modified Eagle's Medium (DMEM) modified to contain 4 mM L-glutamine, 4500 mg/L glucose, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $20.00 Non-Profit:   $20.00

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    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Eagle's Minimum Essential Medium (EMEM) (ATCC® 30-2003)

    ATCC® Number: 30-2003
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: Modified to contain Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate. 

    For-Profit:   $19.00 Non-Profit:   $19.00

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    Product Format: 500 mL
    Comments: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004)

    ATCC® Number: 30-2004
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) contains 2 mM L-glutamine and 1500 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments: NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005)

    ATCC® Number: 30-2005
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Iscove's Modified Dulbecco's Medium (IMDM) contains 4 mM L-glutamine, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate.

    For-Profit:   $22.00 Non-Profit:   $22.00

    Preview Product

    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • Leibovitz's L-15 Medium (ATCC® 30-2008)

    ATCC® Number: 30-2008
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    Leibovitz's L-15 Medium contains 2 mM L-glutamine and no sodium bicarbonate.

    For-Profit:   $23.00 Non-Profit:   $23.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This formulation is designed to be used without additional CO2 in the atmosphere of cell cultures.
  • McCoy's 5A Medium (ATCC® 30-2007)

    ATCC® Number: 30-2007
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications:

    McCoy's 5A Medium is modified to contain 1.5 mM L-glutamine and 2200 mg/L sodium bicarbonate.

    For-Profit:   $18.00 Non-Profit:   $18.00

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    Formulation: Formulation
    Product Format: 500 mL
    Comments:

    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 2.2 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.


  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.00 Non-Profit:   $25.00

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  • RPMI-1640 Medium (ATCC® 30-2001)

    ATCC® Number: 30-2001
    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: RPMI-1640 medium modified to contain 2 mM L-glutamine, 10 mM HEPES, 1 mM sodium pyruvate, 4500 mg/L glucose, and 1500 mg/L sodium bicarbonate, for use in incubators using 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.

    For-Profit:   $20.00 Non-Profit:   $20.00

    Preview Product

    Formulation: Formulation
    Product Format: 500 mL
    Comments:
    NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
  • VeroPlus SFM (ATCC® ACS-4001)

    ATCC® Number: ACS-4001
    Quantity: 500 mL
    Storage: Store product at 2°C to 8°C. Do not freeze.

    For-Profit:   $40.00 Non-Profit:   $40.00

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    Product Format: 500 mL
    Comments:

    Each lot of VeroPlus SFM is tested functionally for growth characteristics and morphology with Vero cells. Additionally, the media is assayed for pH, osmolality, endotoxins and sterility.

    Supplementing with L- Glutamine or L-alanyl-L-Glutamine is required before use.

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