|The addition of peptone following transfection has been shown to increase extracellular protein expression yields in transient transfection protocols.
HEKPlus Boost Solution should be added post transfection to a final concentration of 10% in complete HEKPlus SFM. The solution contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus Boost Solution also contains human transferrin and recombinant human insulin.
This solution has been optimized for use with ATCC® HEK293T/17 SF suspension cells (ATCC ACS-4500), HEKPlus SFM medium (ATCC ACS-4002) and GeneXPlus transfection reagent (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.