GeneXPlus Transfection Reagent (ATCC® ACS-4004)

Product Format 1 mL
Storage Conditions -20°C

Transient transfection, stable transfection, gene expression, protein production, genetic manipulation.  Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266).


This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.


GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCC ACS-4500) and HEKPlus SFM (ATCC ACS-4004) to reproducibly express a wide range of proteins at high levels.

Intended Use

This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use.

Biosafety Level N/A
Introduction GeneXPlus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Quality Control Specifications

Physical characterization of reagent components is performed using mass spectrophotometry and nuclear magnetic resonance (NMR) for identification and determination of purity. GeneXPlus is tested for performance using a luciferase reporter assay in HEK293 cells.



Physical Characterization


Visual Inspection


Functional Assay

>75 ng luciferase/well


2.5 mg/mL in 80% ethanol

*Please consult the Certificate of Analysis for lot-specific test results.

Basic Documentation
Other Documentation
  1. HEKPlus Protein Expression System Parameters
    Cell density at transfection, ratio of DNA to GeneX
    Date Updated: 3/27/2014
  2. GeneXPlus transfection reagent
    Date Updated: 3/27/2014
  3. HEKPlus Protein Expression System vectors
    Expression vectors containing the SV40 origin of replication should be used. These vectors permit more plasmid copies to persist in the transiently transfected cells.
    Date Updated: 3/27/2014
  4. HEKPlus SFM supplements
    Complete culture medium is prepared by adding L-glutamine to the HEK
    Date Updated: 3/27/2014
  5. HEKPlus Protein Expression System time for optimal protein expression
    It usually takes 2 to 3 days to see optimal expression of intracellular proteins and 6 days for optimal expression of secreted proteins. It is recommended that a time course be performed for...
    Date Updated: 3/27/2014
  6. Advantage of using HEKPlus Protein Expression System
    The HEK
    Date Updated: 3/27/2014
  7. HEKPlus Protein Expression System advantages
    The HEK
    Date Updated: 3/27/2014
  8. HEKPlus Protein Expression System to express hard-to-express proteins
    Yes. The HEK
    Date Updated: 3/27/2014
  9. HEKPlus Protein Expression System Scale
    The HEK
    Date Updated: 3/27/2014
  10. HEKPlus Protein Expression System secreted proteins
    It is recommended to collect cell supernatants at day 6 post-transfection. However, one may want to perform a time course study to determine optimal time for collection post-transfection for each...
    Date Updated: 3/27/2014
  11. HEK293T/17 SF cells are clumping
    No. Small aggregates are easily dispersed with minimal pipetting and do not affect the performance of the cell line.
    Date Updated: 3/27/2014
  12. Replenishing medium for HEK293T/17 SF cells
    Yes. Incomplete media changes affect the cell viability and recovery. Spent medium should be completely removed and replaced with fresh medium every 2 to 3 days.
    Date Updated: 3/27/2014
  13. Media volume for HEK293T/17 SF cells
    For protein expression, it is important to have adequate shaking in the culture vessel. We usually add culture medium to 20% volume of a shaker flask.
    Date Updated: 3/27/2014